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The results show that the architectures present negligible nonspecific adsorption and high protein binding activity with the max value of 120.1 mg/g.
We have designed a bradykinin analog, retro-inverso bradykinin (RI-BK), which is characterized by resistance to proteolysis and high binding activity with the bradykinin type 2 (B2) receptor.
Moreover, both WT and mutant mouse spinal cords showed GFAP binding activity with the Myo Va head region (Fig. 1E, lanes 1, 2).
RNA gel-shift analysis (Fig. 3A) shows a significant increase in RNA binding activity with the increased time of infection.
This specific hinge-like structure of TSP1 domain may play a vital role in binding activity with the receptors.
However, the small difference in stability would not account for our failure to detect DNA binding activity with the Ben deletions.
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The receptor was verified to show similar binding activities with the receptor expressed in other hosts.
From measurements of uptake and binding activities with the Gln42Asn and Gln42Leu mutants (Fig 3 and Supplementary Table S1), this residue would appear to play an important role in the mechanism of Mhp1, but the basis for this is not obvious from the crystal structure or molecular dynamics simulations.
The experiments to this point had been performed to localize the Fn-binding activity with the FlpA protein.
The early researches have reported that only the C-terminal domain in Group I has the sequence-specific DNA-binding activity with the W-box, and N-terminal WRKY domain shows weaker binding activity.
The C-terminal 165 175 residues of Elba1 and Elba2 retain DNA-binding activity with the same sequence-specificity as hetero-tripartite Elba complex when they are dimerized by GST moieties.
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