The use of MQ-water, instead of buffer, is deemed justified as in this work we are interested in the binding of the enzymes, not their activity which can be strongly affected by pH.
These C-terminal sequences may contribute to the autoinhibition of the kinase activity, which can be counteracted by binding of other components.
It can be expressed as an ∼50-residue fragment that retains DNA binding activity, which has allowed structural and biophysical analysis.
Catalytically inactive LwaCas13a maintains targeted RNA binding activity, which we leveraged for programmable tracking of transcripts in live cells.
t-Darpp overexpression led to increased CREB binding activity, which was also reversible by Darpp-32.
In contrast to C. arabica, C. canephora has more proteins annotated as containing binding activity, which is extended for the binding activity branch child terms of nucleic acid binding, DNA and RNA binding activities, transcription regulation and transcription factor activities.
The GO analyses showed that the most common molecular function was binding activity, which included ion binding, protein binding, carbohydrate binding, pattern binding, cell surface binding, and lipid binding.
Allosteric inhibition or activation: the enzyme has additional binding sites for specific inhibitors or activators which can change the conformation resulting in a change of the enzyme activity.
Mutational studies have also shown that the direct Tat:7SK RNA interaction does not require Tat's pTEFb-binding capacity, but is solely dependent on Tat's RNA-binding activity, which is attributed to its RNA binding-domain (RBD, G48-R57 14. G48-R57 14
The −1195 A→G polymorphism creates a c-MYB binding site, which can activate COX-2 expression, and displays a higher promoter activity [15].
