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As shown in Fig. 3B, no differences in Ku DNA ends binding activity were observed during adipocyte differentiation.
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Constitutive activation of AP-1 leading to its high binding activity was observed in most of the esophageal tumors, irrespective of their clinical stage and histo - pathological grade; whereas normal adjacent tissues showed low or no AP-1 activation.
Notably, little to none DNA binding activity was observed with nuclear extracts of tumor tissue.
Note, no HNF6 DNA binding activity was observed with control Caco-2 cell cultures (see Fig. 1C).
The best binding activity was observed when the ratio of RelB/RelE reached 2∶1 (Fig. 2B, lane 6).
No binding activity was observed for the other two RelB-like antitoxin proteins (RelB and RelF), even at very high protein concentrations (Fig. 2, 3).
A similar loss of DNA binding activity was observed upon addition of the iron chelator bipyridyl to holo NsrR (data not shown).
Hence, E-box binding activity is observed in the Y1H and in fluorescence studies under more diverse buffer conditions for the more stably folded ArntbHLH-C/EBP than for ArntbHLH.
No significant differences between alcoholics and controls in the DNA binding activity of p50 homodimer and constitutively active NF-κB were found in the MC although a trend (Student's t-test; T1 22 = 2.02, P = 0.055) for decrease of the total NF-κB DNA binding activity was observed in alcoholics (Table S2).
Increased ARE-DNA binding activity was observed maximally at 1 h with 300 μM of pantoprazole.
The strongest binding activity was observed with A. glaucophylla which has a PPARγ IC50 of 807±99.6.
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