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In DG-deficient neural stem cells (DGKO), IIH6C4 immunoreactivity and laminin binding activity were not detected at ∼120 kDa, indicating the absence of α-DG (Figure 2A).
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Antigen binding activity was not compromised by altered glycosylation or Fc mutagenesis, whereas Fc binding to CD16a was significantly enhanced in the order: non-core fucosylated/Fc-mutated double-engineered ≫ Fc-mutated ≥ non-core-fucosylated > wild-type IgG1-Fc.
Since the DNA binding activity was not reduced, E protein levels were unlikely diminished.
Actin filament binding activity was not crucial for the recruitment of lasp-2 molecules to focal adhesions.
Transgenic flies carrying the TBPHF/L150 152 construct under UAS sequences were expressed in neurons using elav-GAL4 and it was found that TBPH without RNA binding activity was not able to rescue the TBPH loss of function phenotypes compared with endogenous TBPH, although the intracellular localization of these constructs was similar in both the cases (Figure S5A).
Loss of binding activity was not attributable to regeneration conditions used between binding reactions (i.e., conditions promoting complete remove of analyte prior to subsequent binding reaction analysis).
YY1 occupancy was detected at this region in both cell lines, indicating that YY1 DNA binding activity is not entirely absent in HS578T cells.
The electrophoretic mobility shift assay showed that IL-1-induced NF-κB binding activity was not changed by rosiglitazone, while IL-1-induced AP-1 binding activity was reduced.
In addition, biological activity assays of such glycoengineered mAbs showed that their antigen binding activity was not altered but significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC) effect [ 70, 71].
In contrast, endogenous ES cell KLF4 DNA binding activity was not detected using the p18INK4c promoter CACC box sequence, although recombinant KLF4 was shown to bind to this probe (data not shown).
B: binding motif; E: Expression difference statistically significant; TCA: Operon functions in TCA eArcA -P) binding activity was not obseArcA -P EMSA and sequence was not used for constructing the weight matrix Among the tested PCR sequences in EMSA with phosphorylated ArcA, 20 showed ArcA(-P) binding activity.
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