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DNA binding activity was verified by EMSA using the sequence: 5′ GCCACGGCCCAGGAAGTGACTCACCCACCCTGATG as previously described [ 5].
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Trypsin activity was verified using a synthetic substrate.
The absence of NaGlu activity was verified by enzyme assay in brain extracts.
CHIP E3 activity was verified in these assays by CHIP auto-ubiquitinylation (Fig. 3B).
Genotoxic activity was verified in PBMC by comet assay.
Gel viscosity and anti-HIV activity were verified for each Lot prior to in vivo use.
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To verify that the binding activity was due to the presence of Pax-5 we performed a super-shift experiment using a Pax-5 specific antibody, a control antibody or no antibody in the binding reaction.
NF-κB binding activity was also increased markedly.
Pleasingly, simultaneous binding activity was confirmed.
DNA binding activity was assessed by EMSA.
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