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Antigen binding activity was not compromised by altered glycosylation or Fc mutagenesis, whereas Fc binding to CD16a was significantly enhanced in the order: non-core fucosylated/Fc-mutated double-engineered ≫ Fc-mutated ≥ non-core-fucosylated > wild-type IgG1-Fc.
Since the DNA binding activity was not reduced, E protein levels were unlikely diminished.
Actin filament binding activity was not crucial for the recruitment of lasp-2 molecules to focal adhesions.
Transgenic flies carrying the TBPHF/L150 152 construct under UAS sequences were expressed in neurons using elav-GAL4 and it was found that TBPH without RNA binding activity was not able to rescue the TBPH loss of function phenotypes compared with endogenous TBPH, although the intracellular localization of these constructs was similar in both the cases (Figure S5A).
Loss of binding activity was not attributable to regeneration conditions used between binding reactions (i.e., conditions promoting complete remove of analyte prior to subsequent binding reaction analysis).
In contrast, endogenous ES cell KLF4 DNA binding activity was not detected using the p18INK4c promoter CACC box sequence, although recombinant KLF4 was shown to bind to this probe (data not shown).
Similar(49)
In DG-deficient neural stem cells (DGKO), IIH6C4 immunoreactivity and laminin binding activity were not detected at ∼120 kDa, indicating the absence of α-DG (Figure 2A).
YY1 occupancy was detected at this region in both cell lines, indicating that YY1 DNA binding activity is not entirely absent in HS578T cells.
In the absence of dimerizer STAT1 DNA-binding activity was not detectable, further substantiating the non-leakiness of the system.
RNA-binding activity was not compromised in a deletion mutant lacking the N-terminal region, showing that this binding was dependent on structures located within the catalytic portion of the molecule.
Both sense and antisense repeat RNA were observed to directly interact with hnRNP F, hnRNP A1, ALYREF, and SRSF2 proteins although the RNA-binding activity was not equal in all cases and for hnRNP A1 was relatively low (Fig. 4).
More suggestions(20)
binding mobility was not
binding ability was not
binding region was not
binding deal was not
binding equilibrium was not
binding pocket was not
binding pattern was not
binding site was not
binding affinity was not
binding mode was not
binding protein was not
binding activity were not
binding energy was not
binding partner was not
binding efficiency was not
binding motif was not
binding constant was not
binding preference was not
binding accord was not
binding model was not
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