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Finally, peroxisome proliferator activated receptor gamma (PPARγ) binding activity was measured to check the binding affinities of both TGZ and TSN.
To analyze the cause of decreased integrase activity of the mutants, the substrate binding activity was measured by electrophoretic mobility shift assay (EMSA).
Nuclear extracts from the cells were prepared and p65 DNA binding activity was measured using the TransAM™ NFκB kit according to the manufacturer's instructions (Active Motif, Carlsbad, CA).
MNCs isolated and NF-κB DNA binding activity was measured as described previously (1).
NF-κB DNA binding activity was measured using the Gel Shift Assay System (E3300; Promega) per manufacturer's instructions.
Nuclear extracts were isolated and p65 DNA binding activity was measured via an ELISA (Active Motif) according to manufacturer's guidelines.
Similar(48)
In the ligand-binding assay both ERa and ERb are determined (i.e., the sum of their binding activity is measured).
PPARγ-binding activity was measured using a FP (fluorescence polarization) assay, and the active molecule was characterized using HPLC and MS. Silica gel (30 70 μm, 60 Å) was purchased from Grace Davison Discovery Sciences.
The residual antigen-binding activity was measured by ELISA using plates coated with oocyst extract of E. tenella.
Nfatc1 and NF-κB (p65) DNA binding activities were measured using a TransAM transcription factor enzyme-linked immunosorbent assay (ELISA) kit (Active Motif, Carlsbad, CA).
Transcription factor DNA-binding activities were measured using Transcription Factor Activation Profiling Plate Array II (Signosis, Sunnyvale, CA, USA) in nuclear extracts prepared from human ATII cells according to manufacturer's recommendations.
More suggestions(16)
binding activity was calculated
binding activity was indicated
binding pocket was measured
binding activity was verified
binding isotherm was measured
binding activity was increased
binding density was measured
binding protein was measured
binding potential was measured
binding activity was affected
binding activity was expected
binding globulin was measured
binding strength was measured
binding intensity was measured
binding activity was confirmed
binding time was measured
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