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In the results of both DAPA and ChIP, C/EBP α-DNA binding activity was induced by AA, whereas the assays using Sp1 and LP1 consensus binding sequences exhibited no obvious variation compared with the control (data not shown).
NF- κB binding activity was induced by radiation alone in both PC-3 and LNCaP cells.
Similarly, the expression of pathogenesis-related 4 possessing chitinase binding activity was induced in A24 and W2; whereas its expression was suppressed in A2.
Despite the reduction in SREPB1 protein level triggered by fibroblasts in MDA-MB-231 cells, an increase rather than a decrease in the transcription factor DNA binding activity was induced in these cells especially by CAFs.
Similar(56)
Electrophoretic Mobility Shift Assay (EMSA) was used to assay whether IRF-1 promoter binding activity is induced in IFN-γ-treated cells.
Figure 3A demonstrates that NF- κB DNA-binding activity was induced by 5-FU in both a time- and concentration-dependent manner in both H630WT and RKOWT cell lines, whereas exposure of these cells to 30 μM 5-FU up to 24 h had no effect on the expression levels of both NF- κB p50 and p65 proteins in the tested cell lines.
NF κB DNA binding and transcriptional activity was induced by dFdC treatment.
RON/Stk activity is induced by binding of its only known ligand, macrophage stimulating protein (MSP) [ 21, 22].
Large scale changes in transcriptional activity are induced when the DNA binding domain is deliberately designed to have lower binding specificity.
Its transcriptional activity is induced by the binding of endogenous and synthetic lipophilic ligands, which has led to the determination of many roles for PPAR-γ in pathological states such as type 2 diabetes, atherosclerosis, inflammation, and cancer.
ConI showed a stronger apoptosis-inducing activity than ConII, similar to their carbohydrate-binding activities as described in the previous study, we found that ConI and ConII could strongly induce apoptosis in the human T-cell lines and Jurkat cells, and that their apoptotic activities are induced via lectin-carbohydrate interactions [ 36].
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