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However, the effect of genistein combined with radiation on induction and nuclear translocation of p21WAF1/Cip1, in addition to the observed increase in cells arrested in G2/M phase, may not occur via an NF-κB-dependent mechanism, as NF-κB DNA binding activity was in fact inhibited by the combined treatment.
Similar(59)
TREM2 binding activity was detected in multiple cells in the region immediately surrounding amyloid plaques.
Similarly, the expression of pathogenesis-related 4 possessing chitinase binding activity was induced in A24 and W2; whereas its expression was suppressed in A2.
As shown in Fig. 3 A, NF-κB DNA binding activity was low in both undifferentiated and decidualizing HESCs and largely unaffected by H2O2.
However, c-Myc induced expression and DNA binding activity was detected in several EBER1 tumours.
NF-κB DNA binding activity was investigated in WT, IKKα −/−, and IKKβ −/− cells.
As shown by EMSA, NF-κB DNA binding activity was enhanced in nuclear extracts of TCR stimulated HIF-1α deficient T cells (Fig. 4A).
Strikingly, the electrophoretic shift resulting from this phosphorylation occurred when CIN85 binding activity was restored in mTTP, with the T302P mutation, and the electrophoretic shift was lost from hTTP after mutating the CIN85 binding site.
One possibility is that the modern HNF4αLA pair accurately reflects the primitive receptor-ligand pair, which would suggest that ligand binding activity was acquired in advance of the ability to modulate transcription.
Anti-tumour binding activity was found in 12 supernatants.
No such binding activity was detected in extracts from epithelial cells (Data not shown).
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