Sentence examples for binding activity was determined from inspiring English sources

To further explore whether active NF-κB can bind to its target DNA sequence and activate gene transcription in response to CCN2 stimulation, NF-κB DNA binding activity was determined by EMSA following incubation of HSC nuclear protein extracts with P-labeled NF-κB oligomers containing NF-κB/CBF1 binding sites.

NF-κB binding activity was determined using the Light Shift Chemiluminesencent EMSA kit (Pierce, Rockford, IL).

DNA binding activity was determined by EMSA using a consensus NF-κB IRDye-labeled oligonucleotide probe (LI-COR).

HIF-1 DNA binding activity was determined in RA FLS nuclear extracts.

NF-κB DNA binding activity was determined by transcription factor assay.

The NF- κB/DNA binding activity was determined with LightShift Chemiluminescent EMSA kit (Thermo Scientific, Rockford, IL, USA).

The FA percentage content in A549 membrane phospholipids, content of COX-2, level of PPAR γ, and NF- κB binding activity were determined.

NFκB-RelA DNA-binding activity was determined by a TransAMTM NFκB-RelA kit (Active Motif) using 5 µg of nuclear extract proteins according to the manufacturer's instructions.

HIF-1 and -2, VEGF, and VEGFR-1 and -2 protein levels were determined using total tissue lysates, whereas HIF-1 DNA-binding activity was determined using nuclear extracts.

The NF- κB DNA-binding activity was determined by EMSA.

NF-κB DNA-binding activity was determined by gel mobility shift assay while NF-κB response gene expression was evaluated using a luciferase reporter.