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Pleasingly, simultaneous binding activity was confirmed.
The efficacy of the K44H mutation was confirmed by the lack of auto-phosphorylation activity of GST-ULK3 K44H in an in vitro kinase assay, and retention of ESCRT-III binding activity was confirmed by Y2H.
The specificity of AP-1 binding activity was confirmed by re-testing the samples for Oct-1 which is ubiquitously active in all cells and was used as an internal control by performing cold competition assay with 100-fold molar excess of homologous AP-1 and a heterologous Oct-1 cold probe.
Similar(57)
Absence of STAT5b nuclear translocation and DNA-binding activity was confirmed by EMSAs (Figure 5E), and the ability of the anti-STAT5b antibody to recognize phosphorylated STAT5b was checked by performing immunoblots with nuclear extracts from GM-CSF-treated murine neutrophils (data not shown), in which STAT5b is known to be rapidly phosphorylated and translocated to the nucleus [28].
Their biological activity was confirmed by screening their binding to ganglioside GT1b by ELISA.
The change in HK activity was confirmed to reflect changes in HK2 rather than HK1 binding (Pasdois et al., 2012).
Remaining etorphine activity was confirmed to be due to activity at KOP receptors by performing etorphine-stimulated GTPγS binding in the presence of the KOP receptor antagonist, norBNI.
Luciferase activity was confirmed via bioluminescence imaging.
Catalytic activity is confirmed by cyclic voltammetry.
The integrity of the clones was confirmed by sequencing and the binding activity was demonstrated by ELISA using both the SAI/II antigen and S. mutans cells.
NF-κB binding activity was also increased markedly.
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