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Because the mechanism by which acetylation of WRN stimulates its catalytic activities is unknown, we examined the effect of acetylation on WRN DNA binding activity using a forked-duplex substrate [30] and a gel-shift assay.
Purified scFv's were tested for their binding activity using a sandwich ELISA.
The nuclear extracts were collected for STAT3 DNA binding activity using a STAT3 filter plate assay as described under Materials and Methods.
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Improvement of subtype selectivity of an inhibitor's binding activity using the conformational restriction approach has become an effective strategy in drug discovery.
To map the key regions of APIP12 required for its interaction with AvrPiz-t, we tested the binding activity using four different forms of APIP12 in a Y2H assay.
Nuclear extracts were prepared, and 15 μg of proteins was analysed for DNA binding activity using the HIV-LTR tandem κB oligonucleotide as a probe for NF-kB (Jacque et al, 2005).
The most active compound d4 was analyzed for heme binding activity using UV spectrophotometer.
Nuclear extracts were assayed for NF-kB binding activity using the consensus sequence 5'-AGT TGA G GG GAC TTT CCC AGG C-3' labelled by [g-32P]-dATP and T4-kinase to a specific activity more than 5 × 10 cpm/μg.
Non-fucosylated and fucosylated anti-CD20s are composed of amino acid sequences identical to that of rituximab, and thus it was confirmed that they exhibited equal CD20 binding activity using the antigen-binding ELISA [ 20].
To investigate whether the recombinant canine sTfR has the ability to bind to CPV and VP2, we used CPV-2a strain and the recombinant VP2 (prepared in our lab) to test sTfR binding activity using ELISA method.
The extract was used for determination of the binding activity using NF-κBp65 consensus sequence: 5′-AG TT GA GG GG AC TT TC CC AG GC -3′ which was obtained from BIOLINE Inc. (Taunton, MA, USA).
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