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Consequently, a number of putative domain binders with both high affinity and specificity were identified, from which the Src SH3 domain was selected as a case study and tested for its binding activity towards the sacum peptides.
IgG showed threefold lower dose-dependent binding activity towards the AgNPs than the hMT1A.
Following gene synthesis, we expressed and purified their constituent THAP domains and tested their binding activity towards the respective Galileo TIRs.
Furthermore, both the scFvs bound weakly to PEP 1-12, in which EYM is located at the COOH-terminus, but the scFvs binding activity towards the peptides increased with the shift of the position of the presumed epitopes towards the NH2-terminus.
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The binding activity towards RhoAQ63L of scFv C1-N1N2 crudextractct and the four elutions fractions were tested and confirmed by ELISA (Fig 6B).
Using similar reasoning, as the ZF2 mutant retains DNA binding activity towards SSBs, one would expect attenuated NAD degradation and TRPM2-dependent Ca2+ transients, particularly at lower levels of O/N stress.
Interestingly, rCfLec-1 exhibited strong binding activity towards PGN in vitro, but PGN stimulation significantly down-regulated the mRNA expression of CfLec-1.
Our previous studies using the engineered monomeric 14-3-3ζ 14-3-3ζ 14-3-3ζ 14-3-3ζm 14-3-3ζm 14-3-3ζmained binding activity towardshowedcthatphosphorylated 14-3-3ζ 14-3-3ζ 14-3-3ζon, it prevented heat-induced aggregation of monomersubfretained1.
By clustering in lipid raft membrane microdomains [11], [44], the oligosaccharide chains of the GSLs exhibit strong binding activities towards their ligands [45].
The binding activity of PdhR towards the binding sites in front of the genes glcD, mraZ and metB were also analysed by electrophoretic mobility shift assays.
(B ) The binding activity of Spy variants towards Im7 L53A is4A is expressed as their dissociation constant (KD) to the client; smaller values indicate tighter binding.
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