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It is possible that the interaction of ACF with DNA may alter the structure of the DNA affecting the DNA binding activity of various proteins.
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To compare the relative intensities of CRM1 bands to yield an estimate of binding activities of various NES mutants, SDS/PAGE gels were dried and scanned with an Epson V300 scanner and the images analyzed with ImageJ software.
Using these models, we studied (1) the structural relationship between the ligand-binding domains (LBDs) of ERs and ARs of human and medaka, and (2) whether medaka ER and AR can be potential models for studying the ligand-binding activities of various agonists and antagonists of these receptors by docking analysis.
The results clearly indicated that glycans on the protein can be identified by their selective binding activity to various lectins.
We estimated the binding activity of oligonucleotides by incubating the same amounts of nuclear protein and various oligonucleotides.
Binding activity of dust samples.
The inherent DNA-binding activity of Smads is further regulated by various transcriptional activators or repressors which regulate gene transcription [ 28].
TCP20 (also known as SPBP- streomelysin-1 platelet-derive growth factor responsive element binding protein) enhances the activity of various transcription factors, including c-Jun [ 15].
New-generation (atypical) antipsychotics (e.g., clozapine), despite their activity in reducing dopaminergic activity through dopamine D2 receptors blockade, have binding activity at various others receptors, including a higher affinity for the serotoninergic 5-HT2Areceptors (high 5-T2A/D2 binding ratio) involved in the treatment of positive and negative symptoms [ 53, 54].
To confirm further this hypothesis, we tested the ATP-binding affinity and ATPase activity of various Hsp90α phosphorylation mimics or deficient mutants at Thr, Thr and Thr.
However, little information is currently available on the binding kinetics and relative biological activity of various VEGF inhibitors.
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