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To assess if overexpression of MTB clpP1P2 is really due to transcriptional activation by Rv2745-S, we sought to interrupt the DNA binding activity of this protein.
In parallel, a decrease in the DNA binding activity of this transcription factor was observed.
As shown in Figure 11A Western blotting experiments with nuclear extracts of tumor tissue revealed highly significant repression of HNF4alpha, while DNA binding activity of this Zn-finger protein was evidenced at gene specific promoters of EGF, Foxc1, Nfsf1and Defrc6.
The intensity of the C/EBPα-DNA complex, reflecting the binding activity of this nuclear factor to its consensus sequence, enhanced from 4 to 11 h and returned to baseline values beyond this time-point (Fig. 6A).
Given that C2F lipid membrane interaction is calcium-independent, the exact reason for the calcium binding activity of this domain is unclear.
While this might be expected to negatively effect phosphate binding, recent high-resolution structural studies of S. pombe Brc1 bound to a target phosphopeptide provide a possible explanation for the phosphopeptide binding activity of this variant.
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This value would indicate the binding activity of the test mAb compared to that of the standard.
This was accompanied by increased DNA binding activity of these C/EBP isoforms and decreased DNA binding activity of C/EBP-alpha.
HNE also decreased the binding activity of the NF-κB subunit p65 to its target DNA sequence.
Here we have determined the histone methyl-lysine binding activity of the PHD fingers present within the Saccharomyces cerevisiae proteome.
Ujita M, Shinomura T, Ito K, Kitagawa Y, Kimata K. Expression and binding activity of the carboxyl-terminal portion of the core protein of PG-M, a large chondroitin sulfate proteoglycan.
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