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HNE also decreased the binding activity of the NF-κB subunit p65 to its target DNA sequence.
DNA binding activity of the NF-κB subunit p65 was measured from cell lysates using a TransAM® Transcription Factor ELISA Kit (Active Motif, Carlsbad, CA, USA).
Ujita M, Shinomura T, Ito K, Kitagawa Y, Kimata K. Expression and binding activity of the carboxyl-terminal portion of the core protein of PG-M, a large chondroitin sulfate proteoglycan.
A competitive binding assay showed that the relative binding activity of hHP-1 was approximately 25% binding activity of the original murine antibody.
The uncomplicated synthesis and the binding activity of the molecule disclosed here represent an attractive alternative to develop new compounds targeting the protein protein interaction of XIAP/caspase9.
The binding activity of the keto form analogue of curcumin to Aβ aggregates was found to be much weaker than that of curcumin derivatives with keto-enol tautomerism.
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The DNA-binding activity of the PD domain is sufficient to induce ectopic eyes in Drosophila through activation of the retinal determination gene network.
Yield and antigen-binding activity of the antibody constructs were compared after standard lab-scale periplasmic expression in Escherichia coli.
The removal of the unpaired cysteine resulted in a significant increase in antigen-binding activity of the crude periplasmic extracts.
The subsequent injections of new IgG samples showed that such regeneration procedure has not essentially affected the level of immunoglobulin-binding activity of the immobilized SPA-Cys.
This post-translational modification interferes neither with the subcellular localization nor the DNA-binding activity of the protein.
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