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Bivalent or bispecific binding activity of proteins has been mainly achieved by assembling two or more domains in a single molecule.
Our server enables fast calculations of ribonucleoprotein associations and predicts RNA binding activity of proteins with high accuracy, thus resulting in a powerful tool for designing new experiments.
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Since the largest portion of the activation effect occurred between −474 and −823 bp of the promoter (Fig. 2C), we evaluated the DNA binding activities of proteins bound to the putative consensus sequences within this region using gel shift analyses.
One of the challenges in molecular biology consists in improving the structural, functional properties or binding activities of proteins.
Nevertheless, our data represent the first evidence that the actin-binding activity of proteins of the spectrin superfamily can affect postsynaptic sensitivity to released neurotransmitter.
Furthermore, to investigate the intracellular DNA binding activity of activator protein (AP -1.
When we examined the endogenous binding activity of these proteins in A549 and NCI-H460 cells that showed relatively high expression of the Snail1 protein in comparison with other cell lines (data not shown), IR exposure simultaneously increased Snail1 protein expression and Snail1 binding activity with DNA-PKcs, however, Si-Snail1 treatment of these cells showed reversed effects.
Additionally, functionalizing Au NPs of different sizes with molecules/proteins of different sizes and shapes, such as dodecanethiol, hemin, human albumin and its antibody, we show that the size of nanoparticles can strongly influence the binding activity of adsorbed proteins and, consequently, the sensor functioning.
We make the assumption that the labeling process does not change the binding activity of the proteins.
The DNA binding activity of bHLH proteins is mainly determined by the basic region [ 2].
These results demonstrate that the NURD complex can associate with chromatin independent of the H3 binding activity of MTA proteins.
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