It was found that the binding of HeV G mutants D260A, G439A, K443A and K465A to both m101 and m102.4 was almost completely eliminated, while the binding activity of mutants G449A and D468A was significantly decreased.
The DNA binding activity of the mutant FadR protein (FadRK113) was examined in vitro using gel shift assays.
Based on the analysis using this cell model, the CD81 binding activity of the mutant E2 protein is less than 5% of that the wild type protein (Fig. 2B).
Interestingly, the specific binding activity of Con-anc mutants, namely, Con-anc-L5, Con-ancN/C/L5, and Con-anc-N/C/L5/L6, were greatly increased against L NFP-II (#44), L NFP-III (#45), L NDFH (#46), and A-heptasaccharide (#48), all of which contained fucosyl-GlcNAc, by 20- to 30-fold when compared with the affinity of Con-anc, and by 3- to 7-fold when compared with that of ConI.
To evaluate the binding activity of wild type and mutant scFv anti-p17 with a series of synthetic peptides (GenScript, Piscataway, New Jersey, USA), phage ELISA was set up as described in our previous study [ 6].
EMSA-based assays of binding activities of four mutant FadR proteins, FadRW60G (C), FadRF74G (D), FadRW75G (E) and FadRF74G, W75G (F) in comparison with that of the wild-type version (B).
However, the binding activity of double- and triple-mutant hRNase3 to LMWH decreased more significantly, especially when HBR1 was mutated.
We report here the use of multiple stabilizing substitutions to increase the stability of p16INK4a and furthermore, to restore Cdk4 binding activity of several defective, cancer-related mutant proteins.
We report 7mG excision and DNA binding activities of AlkD mutants, along with a comparison of alkylpurine DNA glycosylase structures.
