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Despite being a major integrator of titin and actin in one of the stiffest structures of the sarcomere, muscle α-actinin shows surprisingly dynamic association with the Z-disk actin cytoskeleton (Sanger and Sanger, 2008), suggesting that its actin and titin binding activity must be dynamically regulated.
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Therefore, their activity must be tightly regulated.
Parameters that constitute disease activity must be clearly defined.
Such activity must be energetically favorable for the cell.
The role of physical activity must be considered.
These criminal activities must be stopped".
Food and activities must be prepared.
Since the mutation His99Ala abolished Zn2+ inhibition of KLK7 activity completely, this metal binding site must be the structural basis of the attenuating or inhibitory effect of Zn2+ and Cu2+ [147].
Since Rnf2 and the PRCs lack inherent DNA-binding activity, other factors must be involved in targeted recruitment to promoters.
The search for the function-determining amino acids continues and after decades of intensive research we still do not know more than that the substrate binding residues must be directly responsible for the mono- and/or diphenolase activity.
"Rules must be binding, violations must be punished, words must mean something".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com