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The final reported residual DNA binding activity is an average of five independent experiments.
Critical for this binding activity is an intact methyl-binding domain at the amino terminus of the MeCP2 protein [ 69].
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Our study suggests that mCpG-dependent TF binding activity is a widespread phenomenon and provides a new framework to understand the role and mechanism of TFs in epigenetic regulation of gene transcription.
Unlike the in vivo assay, the in vitro binding activity is used as a surrogate method to analyze the mAb candidates.
In human endothelial cells, its location on the membrane is affected and its GTP binding activity is inhibited by a high concentration of mevastatin (10 μM) [ 31].
DNA binding activity is confined to a highly conserved ~300-residue ~300-residueregiono as the referredlogy region (RHR), toas is presentheearelhomologymini of NF-κB subunits.
In this case, DNA binding activity is likely limited by a slow rate of dissociation of the GST-ElbaF homodimers.
This suggests that its DNA binding activity is likely controlled via phosphorylation by a histidine sensor kinase.
This specific DNA binding activity is associated with the regulation of a number of cellular processes, including transcription, DNA replication, and site-specific recombination.
However, this DNA binding activity is diminished in both IKKα and IKKβ deficient cells.
10.7554/eLife.00726.017 Figure 3. KLF4's mCpG-dependent binding activity is decoupled from its binding activity to unmethylated motifs.
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