In addition, supershift analysis of C/EBP element binding activity indicates that C/EBPβ is the active isoform involved in the observed TCDD-mediated increase in binding affinity (Fig. 3b, lane 4).
The results of AI-2 binding activity indicated that 5 mg/ml of LsrB (BL21∆luxS) could bind exogenous AI-2 from 2.5 to 40 μM (Fig. 6).
The results of AI-2 binding activity indicated that 5 mg/ml of LsrB (BL21∆luxS) could bind exogenous AI-2 from 2.5 to 40 μM.
Netrin-1 interacted with Fc-CD146 or Fc-UNC5B, whereas Fc alone did not show binding activity, indicating that the extracellular region of CD146 directly binds to netrin-1.
MBP-Head did co-immunoprecipitate with NF-L, while MBP alone or BSA (data not shown) showed no binding activity, indicating that the MBP-Myo Va head interaction with NF-L is specific.
In sharp contrast, neither GAL4 protein nor mouse non-specific IgG showed such binding activity, indicating that TfRscFv-GAL4 fusion protein binding to human gastric cancer cells and human breast cancer cells is through the TfRscFv domain but not GAL4 protein domain.
Stearic (18 0), palmitic (16:0), and myristic (14 0) acids failed to inhibit MSI1 RNA-binding activity, indicating that the ω-9 double bond is required.
Treatment of the WT TMK-1 cells with an IKK inhibitor BAY-117082 strongly suppressed the p65 DNA-binding activity, indicating that a high level of NF-κB transcriptional activity is maintained in gastric cancer cells, even under a steady-state culture condition (Fig S5C of Supporting Information).
However, removal of calcium does not completely abolish activity, indicating that calcium binding at these sites is not essential for activity.
The inability of a primer template that is bound to the polymerase active site to inhibit exonuclease activity indicates that the two activities have independent binding domains.
Previous studies have already shown that ISVP have lost their haemaglutination activity, indicating that their cell binding properties have changed, particularly for red blood cells.
