The overexpression of wild-type IRP1 or IRP1C437S, a mutant with constitutive IRE-binding activity, in human H1299 lung cancer cells did not affect their proliferation in culture, but significantly impaired the growth of tumour xenografts formed upon injection of these cells into nude mice [ 202].
We therefore studied the consequences of functional knockdown of FOXA2 on HNF6 DNA binding activity in the human colon cancer cell line Caco-2.
Recently, full-length HP was expressed and purified using a human cell line and was shown to display Hε binding activity in vitro and in cells.
MEME alignment with the κB sites from the human sequence identified three putative κB sites, but none showed any binding activity in EMSA (Fig. 3).
Using our protocol for fast extraction of nuclear proteins from frozen tissues, we observed similar levels of the NF-κB/p50 homodimer DNA binding activities in extracts of frozen human samples and of rat brain tissues prepared immediately after decapitation, thus demonstrating low if any inactivation of these factors in human samples.
We predicted that W222R and G225D mutations in HA result in a decreased binding activity to both human and avian receptors, particularly to avian receptors, during the evolution of the Fujian-like viruses.
Interestingly, loss of messenger RNA and protein expression of survivin, an inhibitor of apoptosis, as well as decreased STAT3 DNA binding activity was observed in human rhabdomyosarcoma cells treated with FLLL32 [ 42].
Herein, we report substituted styryl chromones as a new class of compounds that exhibit selectivity for ERβ binding at the second binding site of HT and antiproliferative activity in human breast cancer cell line.
In human hepatoma HepG2 cells, chrysophanol, danthron, and rhein also suppressed the DNA-binding activity in a dose-dependent manner, although aloe-emodin showed a moderate effect.
