Sentence examples for binding activity in an from inspiring English sources

For all 27 ETS proteins, DNA binding activity in an EMSA confirmed proper folding (Additional file 2: Figure S1).

Enriched short pili as well as recombinant purified ComGC lacking the first 39 amino acids showed no DNA binding activity in an electromobility shift assay.

To confirm that mutants M-1-2, M-1, and M-2 lost their polyubiquitin binding activity, we tested their binding activity in an ELISA binding assay in which polyubiquitin was adsorbed on a microtitre plate.

This study demonstrated an improvement to these previous reported studies by showing the robustness of the selected Exotoxin A specific MRE in undiluted serum without any base modifications, and it was able to retain a level of binding activity in an environment that was very much different compared to the selection condition.

The CM induced c-Jun accumulation and dephosphorylation and increased AP-1 DNA binding activity in a time-dependent manner.

The presence of GSH-OEt depressed NF- κB-DNA binding activity in a dose-dependent manner in U937-Neo cells treated with 10 ng ml−1 of TNF- α.

Subsequently, we determined that ET-1-stimulates NF-κB p65 binding activity in a time-dependent manner by ChIP-PCR analysis.

Furthermore, the co-expression of C35 and ΔNp73 Δ1–76, which showed no binding activity in a co-immunoprecipitation assay, had no significant effect on the level of apoptosis.

We have tested the four core members of the Gfrα family (Gfrα1 4) and found that they all lack Shh-N binding activity in a COS cell surface binding assay (not shown).

In further in vitro study, thiacremonone (2.5-10 2.5-10 inhibited lipopolysaccharide (LPS, 1 μg/ml)-inhibiteditric oxide (NO) production, and NF-κB transcriptionalipopolysaccharideactivity in a dose dependent manner.

Tumor extracts containing either IRP2wt or IRP2Δ73 exhibited high IRE-binding activity in an electrophoretic mobility shift assay (EMSA) (Fig. 4B).