Sentence examples for binding activity could be from inspiring English sources
The domain 1 binding activity in patient serum was abolished by removing the IgG fraction from the serum and the binding activity could be fully reconstituted with the IgG fraction.
Nonetheless, HNF4α DNA binding activity could be assayed for in EMSA supershift assays and was significantly reduced to 58% after treatment with 8.3 µM (10 µg/ml) cyclosporine (Fig. 3C, 3D).
For these regulated genes, we investigated if within the respective promoter regions where putative indirect OCT4 binding activity could be confirmed, one could also detect an enrichment of known transcription factor binding sites by adopting a de novo motif discovery approach.
However, in both cases residual binding activity could be detected.
Of course, the mRNA level does not necessarily alone determine the TF's actual protein-level [ 42] and binding activity could be influenced by other variables, such as for example post-translational modifications, chromatin differences, and co-factor availability.
As one important determinant of binding activity could be TF availability, we looked at how the TF mRNA expression levels, as measured by RNA sequencing, correlated with the number of peaks.
In contrast to Hsf2+/+ cortices, no HSF1 HSE-binding activity could be detected in Hsf2−/− cortices, when exposed to alcohol in utero (Fig 5D and E).
Using this analysis, quantities of HaloTag-fusion proteins with ligand-binding activity could be directly estimated from the fluorescence intensities of protein bands on SDS PAGE.
STAT1 DNA-binding activity could be detected after 12 h treatment over a range of 5 100 nM rapalog, although, consistent with the protein expression data, the level under these conditions is lower than in WT cells.
