However, there are two major differences between these proteins: first, PML is ubiquitously expressed whereas PRH shows tissue-specific expression; secondly, PML lacks DNA-binding activity and can only function as a co-repressor or co-activator of transcription, whereas PRH has strong DNA-binding activity and can use multiple mechanisms to regulate transcription.
This is mostly consistent with the BH3 profiling assays and it suggests that the ability of Bcl-xL to regulate cell survival during mitotic arrest relies on its BH3-binding activity and can be harnessed pharmaceutically.
The strong reduction in CSF2 mRNA and GM-CSF protein (Fig. 4, 6) after silencing NF90 are likely due to indirect effects of NF90; for example, NF90 may regulate the expression of another RBP that influences CSF2 mRNA levels, or perhaps NF90, which has DNA-binding activity and can function as a transcription factor [ 34], might transcriptionally upregulate CSF2 transcription.
WSSV069 (ie1) can function as a transcriptional regulator, exhibits transactivation and DNA-binding activities and can take advantage of the shrimp STAT pathway to enhance its own expression.
Following metabolism by colonic bacteria to more biologically active metabolites, isoflavones and lignans show weak estrogen receptor (ER) binding activity and, depending on the context, they can act to either mimic or counteract the effects of endogenous estrogen, 17β-estradiol.
Deacetylation of sequence-specific transcription factors can decrease their DNA binding activity and subsequently may repress transcription.
CSD possesses nucleic acid binding activity and is capable of binding to single-stranded DNA/RNA and double-stranded DNA19.
Taken together, our results argue that MPP8CD, TDRD7TD, and JMJ2CTD represent three new domains with specific histone PTM-binding activity and that HEMP technology can be used to identify and easily validate novel chromatin effectors.
Using separate sets of mutations that affected either Imp2 membrane-binding or tubulation activities, we surprisingly found that Imp2's cytokinetic functions rely only on F-BAR membrane binding, rather than tubulation activity, and can be replaced by other nontubulating cytokinetic F-BAR domains.
For the fitness accuracy, the lower energy is associated with the greater binding activity which can also give rise to better drug efficiency.
