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This study elucidates the different regulatory and DNA binding activities of three FruM isoforms on a genome-wide scale and identifies genes regulated by these isoforms.
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EMSA-based assays of binding activities of four mutant FadR proteins, FadRW60G (C), FadRF74G (D), FadRW75G (E) and FadRF74G, W75G (F) in comparison with that of the wild-type version (B).
To further characterise the binding activity of two of the constructs 4D5-8RFP and 4D5-8CIT we used immunofluorescent cell staining as shown in Figure 6.
We examined the haem-binding activity of three ANT isoforms (ANT1, 2 and 3) using recombinant proteins and found that haem bound to all the ANT isoforms similarly (Fig. 1C).
The obvious carbohydrate-binding activity of three different EUL proteins from taxonomically unrelated species strongly indicates that several EUL domains can be considered lectin domains.
To address whether methylated CpG in a particular site interferes with the binding of these transcription factors, we first examined the interfering on binding activities of these two transcription factors by EMSA.
To confirm the differential binding activities of these two constructs seen in IP assays with NF-L, we subjected cytoskeletal fractions from spinal cord to blot overlay assays with 41-326 and 327-505 in equimolar amounts.
mAb-specific domains were used to develop an enzyme-linked immunosorbent assay (ELISA) for characterization of the integrity and binding activity of the three mAbs in the drug product.
Following CCN2 stimulation, a marked nuclear translocation of NF-κB was evident along with a persistent DNA binding activity of its three dimers, and an induction of IκBα phosphorylation and degradation.
Although cadmium accounted for reduced binding activity of all three subtypes in most brain regions, it was subtype 2 that seemed to be its main target, as shown by a very great (p < 0.001) down-regulation in mesencephalic areas such as the stratum griseum central layer.
In contrast, the binding activities of the oligos containing two CpG flanking the AP-1 site were not reduced by methylation in EMSA (Figure 7B).
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