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Despite the extensive in vivo functional studies of the exosome, little information is yet available on the structure of the complex and on the RNase and RNA binding activities of the individual subunits.
Both the ATPase and nucleic acid binding activities of the DEAD-box proteins are functionally connected to their helicase activity.
Binding activities of the purified T3sVHHs were tested by ELISA using antigens Amy, GFP or BSA (as negative control).
In contrast, the binding activities of the oligos containing two CpG flanking the AP-1 site were not reduced by methylation in EMSA (Figure 7B).
E-312 lies in an exposed loop of EIII, where it may contribute to the antigenic and/or putative receptor binding activities of the domain (6 ).
The experimental validation of these ideas as well as an investigation of the DNA binding activities of the SinR-SlrR complex will be the subject of further work.
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HNE also decreased the binding activity of the NF-κB subunit p65 to its target DNA sequence.
Here we have determined the histone methyl-lysine binding activity of the PHD fingers present within the Saccharomyces cerevisiae proteome.
Ujita M, Shinomura T, Ito K, Kitagawa Y, Kimata K. Expression and binding activity of the carboxyl-terminal portion of the core protein of PG-M, a large chondroitin sulfate proteoglycan.
DNA binding activity of the NF-κB subunit p65 was measured from cell lysates using a TransAM® Transcription Factor ELISA Kit (Active Motif, Carlsbad, CA, USA).
A competitive binding assay showed that the relative binding activity of hHP-1 was approximately 25% binding activity of the original murine antibody.
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