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In parallel, we assessed the AP-2 levels and DNA binding activities in all these cell lines.
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Indeed, recombinant IL-18 led to a significant increase in NF-κB DNA binding activity in T-ALL cells (Supplementary Fig S5D and E).
NBT-272 (0.4 μg/ml) treatment for 24 h reduced c-MYC binding activity significantly in all MB cells tested.
It is phosphorylated, leading to calcium binding activity in some, but not all, investigated dehydrins [ 53].
For all 27 ETS proteins, DNA binding activity in an EMSA confirmed proper folding (Additional file 2: Figure S1).
All three Elba proteins are required to reconstitute DNA binding activity in vitro.
For example, Znf114 and Znf416 were identified as new generic methylated-DNA binding proteins, with binding activities to almost all methylated DNA motifs tested in this study.
Although cadmium accounted for reduced binding activity of all three subtypes in most brain regions, it was subtype 2 that seemed to be its main target, as shown by a very great (p < 0.001) down-regulation in mesencephalic areas such as the stratum griseum central layer.
SP-A binding assays using Escherichia coli HB101 were used to validate the binding activity of all SP-A preparations used in this study.
As shown in Figure 6A, the competitive binding activity of all compounds tested is dose-dependent.
30 Significant binding activity was detected in all cell lines tested, including HEK293T cells (Fig. S2b).
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