Sentence examples for binding accessibility from inspiring English sources

To test this hypothesis, we developed novel scoring methodologies to semi-quantify the co-transcriptional binding accessibility of AON target sites.

However, the identification of effective AON target sites remains unsatisfactory for lack of a precise method to predict their binding accessibility.

Subsequently, each nucleotide within the AON target site will be scored for binding accessibility based on whether it is paired in the predicted secondary structures.

The AON target sites of 2 sets of published AONS were scored for co-transcriptional binding accessibility and their scores correlated with the degree of reported AON efficiency and efficacy.

The development of scores at four levels of analysis to semi-quantify co-transcriptional binding accessibility of AON target sites allows their correlation with AON efficacy and efficiency using statistical tests.

Interestingly, exon 57 manifests an overwhelming occurrence of engaged nucleotides (Figure S3 of the Online Supporting Information); hence, it is relatively difficult to locate a suitably long sequence that has ESE activity as well as co-transcriptional binding accessibility that fulfils the four insights (as described above).

Besides co-transcriptional binding accessibilities of AON targets, AON efficiency depends on other factors such as presence of ESEs, stability of AONs (by chemical modifications), thermodynamic considerations, absolute distance of AON target site from 3' splice site, etc.

In an elegant study on the rules determining global nucleosome positioning in yeast, Segal et al. found low nucleosome occupancy at TSS and examined frequencies of TFs for the occurrence of nucleosome occupancy: the yeast NF-Y homologues HAP2/3/5 came on top of the ranking for binding site accessibility by intrinsic nucleosome positioning [48].

The intersubunit motions may serve as a gate to control binding site accessibility.

mRNA folding is expected to interact with ribosome binding site accessibility and modify individual gene expression as demonstrated in E. coli[ 25, 39].

Such biochemical events include sites of transcription factor and micro RNA (miRNA) binding, chromatin accessibility and modifications, DNA methylation, and many other types.