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Phage display technology has been used for exploring novel peptides acting on GPCRs, but it is unclear whether the identified peptides functionally modulate targets because the technology selects peptides based on binding ability but not functional activity to targets.
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Therefore we further investigated whether AtYLMG1-1 has a DNA-binding ability, but we could not get recombinant YlmG proteins by expression in E. coli due to the lethality of the YlmG overexpresser.
The results showed that the protein binding ability is not influenced when they are administrated simultaneously.
Webster stressed that the receptor binding ability was not the only factor that makes the virus so worrying.
Next, the sensitivity of the methylation effect on transcription factor binding ability is not the same at different methylation levels.
Therefore, a subtle difference in Rap1 binding ability might not result in a loss of silencing at that locus.
It has been previously shown that a certain arginine-rich peptide exhibits not only RRE-binding ability but also cell permeability and antagonism of CXCR4, one of the major coreceptors of HIV-1.
Results obtained from the batch binding experiments demonstrated that all of the TIPs have sites that have selective binding ability for theophylline, but not to another structurally similar molecule, caffeine.
Knockdown of FTO specifically affects the RNA binding ability of SRSF2, but not that of SRSF4.
This may affect on the MAb binding ability (not the A-chain toxicity), while we have shown by ELISA and FACS which is not affected.
This idea of lipid selectivity and preferential binding has been corroborated to an extent, in studies which showed that dysferlin C2A could be distinguished from other C2 domains with its unique phosphoinositide binding ability not commonly seen in others [ 44].
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