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Furthermore partially purified polymerases were active in an in vitro replication assay and in promoter binding (Results not shown).
Several other ERK inhibiting compounds that did not cause FN-fibril disruption were found to be ineffective in integrin inactivation as determined by HUTS-4 antibody binding (Results not shown).
Additionally, results indicated that preloading cells with rhSLPI did not impair IL-8 membrane binding (results not shown).
GBP H152C and GBP H152C/A213R labelled with Red Oxazine and Chromis 630 did not show a significant fluorescence change upon glucose binding (results not shown).
Although no crystals could be obtained with NbGAK_2, this Nb showed the highest affinity to GAK target in comparison with all other Nbs tested in direct binding experiments using SPR (Table 1), whereas no rate and equilibrium-binding constants could be determined in a direct binding assay for the fourth Nb due to unspecific binding (results not shown).
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This might indicate that there has been a change-of-function for this domain in the plant lineage, although the newly evolved cysteine residue in the P-loop is not essentially required for Fe/S cluster-binding (result not shown).
Similar results were obtained in 70K binding experiments (results not shown).
Unfortunately, none of them, albeit associated with NLPs, exhibited ligand binding activity (results not shown).
A similar result was obtained with a primer pair covering a smaller region containing the two binding sites (Results not shown).
A single mutation in the C-terminal helix [CXCL8 Δ6E70R)], however, was not sufficient to increase the GAG-binding affinity (results not shown).
Moreover, AAL-2 binds to glycans in a metal-ion-independent manner, whereas Ca2+ is required for GSL-II-binding activity (results not shown).
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