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The corrected fluorescence due to binding (Fbind) was calculated using eq 5.
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The binding constant was calculated to be 4.57 × 104 M−104
Binding energy was calculated by applying CHARMm forcefield.
The substrate binding energy was calculated and the electrostatic energy was found to be the most important term for binding.
An average binding ratio was calculated ± one standard deviation.
Then, the nucleosome occupancy surrounding the binding sites was calculated.
Specific binding was calculated by subtracting nonspecific binding from total binding.
The specific binding was calculated by the total binding (cpm) - nonspecific binding (cpm).
The specific binding was calculated by subtracting the non-specific binding from total binding.
Specific binding was calculated by subtracting the non-specific binding from the total binding.
The specific binding was calculated by subtraction of the non-specific binding from the total binding.
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