Sentence examples for binding DARPins were detected from inspiring English sources

Expression cultures were harvested by centrifugation, lysed, the crude extracts were applied to VEGF-A coated Maxisorp plates, and binding DARPins were detected using an anti-RGSHis antibody (QIAgen)/Anti-mouse IgG-AP conjugate (Sigma) detection system in combination with a 4-NPP (Sigma) detection system as described [ 23].

Binding DARPins were detected using an anti-RGS-His antibody/anti-mouse-IgG-AP conjugate system as described [ 17].

DARPins were detected via an anti-RGS-His6 antibody (Qiagen, 1 5000 in PBS-TB), an anti-mouse-IgG conjugated to alkaline phosphatase (Pierce ,1 10,000 in PBS-TB) and p-nitrophenylphosphate (3 mM in 50 mM NaHCO3, 50 mM MgCl2).

By increasing laser intensity, dimeric and monomeric AcrB species with bound DARPin molecules were detected showing the high affinity binding of DARPin to monomeric AcrB species.

Our results showed that all DARPins tested induced strong signal suppression of more than 50%% (Fig.  1), even when using only 25 pM (monomer) VEGF-A, whereas the isotype controls (i.e. non-binding DARPins) were not affecting the signals.

Binding DARPins were then expressed, purified and characterized by different ELISAs and cellular assays.

DARPin-bound cells were detected 30 minutes after the intravenous injection not only in the peripheral blood, but also in the lymphoid tissue.

DARPin-bound CD4+ cells were detected in the peripheral blood as early as 30 minutes after the injection, decreasing within 6 hours and being almost undetectable within 24 hours.

A second injection was performed as soon as the instrument was ready, at which point 1 µM of each GFP binding DARPin was injected in one of the analyte channels.

After 24 hours, binding by DARPin 57.2, but not by DARPin E3_5, was detected on CD4+ cells (Figure 4C).

The binding was detected by Streptavidin-HRP conjugate.