Sentence examples for binders were isolated using from inspiring English sources
Cells were washed as above and αvβ3 integrin binders were isolated using a Becton Dickinson FACSVantage SE instrument (Stanford FACS facility).
PBMCs were isolated using ficoll gradient centrifugation.
Total RNAs were isolated using TRIzol (Invitrogen).
DNA was isolated using standard extraction protocols.
Total RNA was isolated using Isogen (Wako).
Total RNA was isolated using TriReagent (Molecular Research Centre).
Based on phage ELISA results, the strongest binders were isolated and used for subsequent expression and purification of POM scFv.
About three times more binders were isolated from the panning when using the antigen MUC1 and competition with HT186-D11.
As expected, more unique binders were isolated from the macaque immune library as from the naive human antibody gene library.
PBMCs were isolated by use of Ficoll.
Three binders, B10, B13 and B14, were isolated by selection on the major isoform Bet v 1.0101, while selection on isoform Bet v 1.0102 generated one additional binder (M0418) together with re-isolation of binder B14.
