Much of our research involves cell surface oligosaccharides, biopolymers that contribute to cell surface recognition and cell-cell communication.
Using a human T cell line, gene-modified to express a tumor-specific CIR on their surface, and a human tumor cell line we demonstrated that combining CIR-mediated activation with FACS sorting of CD69+ T cells, it is possible to isolate binders to tumor specific cell surface antigen, with an enrichment factor of 103-fold after two rounds.
Based on an effective interaction between the CIR and the tumor antigen, we demonstrated that combining CIR-mediated activation with FACS sorting of CD69+ T cells, it is possible to isolate binders to tumor specific cell surface antigen, with an enrichment factor of at least 103-fold after two rounds, resulting in a homogeneous population of T cells expressing tumor-specific CIRs.
Flow cytometry was used to identify cell surface antigen.
Specific binders to the virus surface protein hemagglutinin (HA) are of interest for influenza diagnosis as well as for therapy.
However, one must bear in mind that one of the main capabilities of SPR, the analysis of the binding kinetics of single known binders, is lost when analyzing mixtures of (unknown) proteins when it is coupled with MS. Then, SPR can only be used for semiquantitation of all combined binders to the SPR surface.
In this paper, we tested the whole procedure of a HTS-based screening, to isolate binders of native CLDN1 protein, expressed on the cell surface of mammalian cells.
Applications to detection of lymphokines and cell surface antigens.
Engineering cells for desiccation tolerance, unveiling the metabolism of non-growing cells, and engineering the interaction between the cell surface and adhesive polymer binders are fundamental challenges to open the door to vast future applications of biocoatings for environmental sensing and remediation.
As a group of natural glycan binders, lectin is an ideal tool for cell surface glycan profiling.
