STAT family members bind with varying affinities to a canonical palindromic sequence (TTCN2-4GAA) in the promoters of their target genes [ 89].
FnBPs contain multiple tandem fibronectin-binding repeats (FnBRs) which bind fibronectin with varying affinity but it is unclear what selects for this configuration.
Structure function studies on the Mg2+-sensing riboswitches, so-called M-box riboswitches, revealed that in vitro these riboswitches bind different metal ions with varying affinity, but similar cooperativity.
Furthermore, although they are not considered to be immunoglobulin-like repeats, the eleven tandem repeats in FnBPA from S. aureus that are downstream of the fibrinogen- and elastin-binding A region individually bind fibronectin with varying affinities [49].
Besides PCDDs, PCDFs, and some PCBs, a number of structurally divergent compounds bind to the dioxin receptor with varying affinity (Safe 1995).
CagA binds all four PAR1 isoforms with varying affinity [ 72], and the PAR1b-binding region of CagA has been identified as the 16-amino-acid CagA sequence also termed the CagA-multimerization (CM) sequence, which is involved in CagA dimerization [ 73].
All three fatty acids have been reported to bind PPARα with varying affinities in the micromolar range [ 30- 33].
Isakov, N. et al. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity.
They generally bind the 3' untranslated regions (UTRs) of target mRNAs with varying affinity, resulting in mRNA degradation or inhibition of protein translation [ 1].
We have previously reported that the PH domains of several proteins, including beta ARK1, PLC gamma, IRS-1, Ras-GRF, and Ras-GAP, expressed as glutathione S-transferase fusion proteins, can reversibly bind purified bovine brain G beta gamma subunits in vitro with varying affinity.
