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Since OTA can competitively bind with the aptamer due to their high affinity, the presence of OTA will induce the decrease of guanine-rich DNA on the electrode surface, in turn reduces the signal of methylene blue which can specifically adsorb to the guanine units of DNA.
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The methylene blue is used as a tracer and a guanine-rich complementary DNA sequence is designed to bind with the unbound OTA aptamer for signal amplification.
In the proposed method, biotin labeled and free OTA competed to bind with immobilized aptamer onto the surface of a screen printed carbon electrode (SPCE), and percentage binding was calculated.
Methylene blue was applied as a tracer and a guanine-rich complementary DNA sequence was designed to bind with the unbound 17β-estradiol aptamer for signal amplification.
RRM1 bound to the aptamer RNA with an apparent Kd of 75.2 ± 10 nM by FP.
We employed a designed complementary DNA featuring a guanine-rich section in its sequence (cDNA G1) as a detection probe to bind with the unbound anti-human IgE aptamer.
The TMPyP4 molecules in the complex had been identified to bind tightly to the aptamer by intercalation and outside binding.
Here, we design and prepare FSNPs modified with the aptamer Sgc8, which binds tightly and specifically to T acute lymphocytic leukemia cells.
CEA and hemin competitively bound with the dual DNA aptamer while the mixture in a detection cell was incubated for 30 min at room temperature.
To test the influence of dT-spacers on the ability of the St-2-1 apTable (Table 2) to bind to streptavidin, an array was synthesized on hydroxyl-functionalized substrates, with the aptamer sequence on spacers ranging in length from an oligo-dT 1mer to 25mers.
When the target cells competed with cDNA to bind with aptamer, double-stranded DNA was denatured and PtNPs-DNA bioconjugates were released from the ITO electrode, resulting in decreased current response.
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