JEG-3 cells expressing ADAMTS-12 adhered to ECM deposited by proteinase-dead ADAMTS-12 expressing cells and vice versa with high affinity, whereas control LacZ-expressing cells bound with less affinity to ECM deposited by A12FL or A12Mut expressing cells.
Indeed, phospho-mimetic GST-HtsS704D bound with less affinity to multiple phosphoinositides when compared to wild-type GST-HtsS704S (Fig. 7A).
p130Cas-P2 presents an exclusive strong interaction with MLPK3, PSD95, PI3-ß (array I), GRB2L-D1, NE-DLG and NOF2-D1 (array II), and binds with less affinity to amphiphysin, RasGAP (array I), CSKP, BIN1 and EFS.
Similarly to C3G and Cbl, it presents a clear direct association with LCK, Cortactin, Yes1, Abl2, SLK, c-Src, Hck, VAV2-D2, Y124, PEXD, BTK, ITSN-D1 (array I), Abl2, NCK1-D2, OSF, PI3α, Tec, PIG2, VINE-D3 and c-Src, (array II) and it binds with less affinity to Stam, BLK, Abl and CrkL-D1 (Additional file 7).
It is possible that the other metals bind Tau with less affinity and the internal cellular environment does not provide high enough levels of these ions.
enzymes that consume SAM can be expected to bind methionine with less affinity, possibly indicating that the hexacoordinate geometry of the unique iron does not occur as readily in these cases (or more readily exchanges with another molecule of SAM).
Pre-miRs having shorter uninterrupted stem segments might be expected to bind dsRNA binding domains with less affinity, particularly if these proteins compete with each other for binding.
Using competitive radiometric binding assays, we found, as reported previously (Visser et al, 2008), that E4 and E3 bind to ERα with less affinity than E2 and with a small preference over ERβ (Supplementary Table S1).
Carbon monoxide binds to cytochrome oxidase with less affinity than oxygen, so it is possible that it requires significant intracellular hypoxia before binding.
These studies demonstrated that nine different antibiotics that bind strongly to this site in bacteria bind with much less affinity in archaea.
