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RelA/p65 and c-Rel directly bind to two putative Rel-binding site on the Rorc promoter and enhance RORγt expression, whereas none of NF-κB family transcription factors bind to Il17a promotor.
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Using ChIP, it was shown that HIF1α binds to three putative HREs in the Hamp promoter and reduces Hamp expression in both human cells [human embryonic kidney (HEK 293 cells] and mouse liver tissues [ 83].
The putative functional residue Arg346 that interacts with both these compounds is no longer classified as an HCR-13, but the compounds still bind to one other HCR-13pf residue (Table 1).
TRAIL can bind to four membrane receptors.
We further examined whether FOXA1 and AR could bind to the five putative FOXA1-AR binding regions, including the promoter and enhancer regions upstream of the TSS (transcription start sites) of AR target genes such as MYC.
Corticosterone binds to two types of receptors.
AAbs bound to fifteen different retinal antigens.
The ligand TRAIL binds to five receptors.
To determine whether p53 can bind to either of the two putative binding sites in the mouse Tigar promoter, chromatin-immunoprecipitation was carried out in mouse 3T3 cells treated with CDDP to activate p53.
Our ChIP assays showed that both FOXA1 and AR could bind to all the five putative FOXA1-AR-binding regions in MFE-296 cells.
Similar effects, although to a lesser extent, were also observed after Snail1 overexpression, which can bind to only two of the four potential ZEB1-binding sites within the putative promoter (Fig 2B, middle).
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