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Addition of 100 m m KCl to a sample containing 100 m m NH4+ led to a reduction (∼50 %) in the NH4+ integral, thus indicating both that NH4+ and K+ compete for the same binding site and that they bind to this site with similar affinities.
It is quite possible, that KA may also bind to this site or has a different peripheral binding site yet to be elucidated.
Accordingly, the following catalytic sequence is proposed (depicted in Figure 6): It is assumed that PGG2 has a high binding affinity for the peroxidase site of the enzyme and will tightly bind to this site.
The crystal structures of two small inhibitors that bind to this site and of a quinolinone inhibitor, that spans the canonical deep pocket near Pro-1 and the new surface binding site, have been solved.
An electrophoretic mobility shift assay (EMSA) was therefore employed to determine if B-MYB could bind to this site.
AP endonucleases bind to this site and cleave the DNA 5' to the abasic site, forming a free 3'-hydroxyl which is repaired by DNA polymerases.
Similar(34)
Further work to identify the DNA-binding protein that binds to this site will help to elucidate the mechanistic basis of how variants within the MLH1 5′UTR affect expression.
C/EBPβ clearly binds to this site in rat GCs undergoing luteinization (Fig. 3B).
The crystal structures of four of these compounds bound to this site are presented, and reasonable modelled docking modes are suggested for the 5 other scaffolds.
A highly conserved asparagine residue, near the C-terminal end of GGG-containing TM5, is positioned near the three-fold axis, and Na+ and Mg2+ cations were found to be bound to this site (N144 in SaTRIC) in SaTRIC structures (type2a and b; Supplementary Table 2 and Fig. 7a,b).
They were acting as ATP minetics that bound to this site and competed with cellular ATP [ 9, 11– 13].
More suggestions(19)
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