Suggestions(5)
Exact(2)
It is given as the concentration of the RNA fragments which specifically bind to this probe.
In contrast, endogenous ES cell KLF4 DNA binding activity was not detected using the p18INK4c promoter CACC box sequence, although recombinant KLF4 was shown to bind to this probe (data not shown).
Similar(58)
Non-target DNA molecules do not bind to the probes and pass through the system.
Although probes are designed to hybridize to a target transcript, off-target transcripts which share sequence similarity to the probe may also bind to the probes.
CIN85 alone did not appear to bind significantly to this probe (Fig. 8A, lane 14), nor did it appear to alter the binding of the various concentrations of hTTP (Fig. 8A, lanes 9 11).
This gives 20144 different TF-probe pairs where we can classify whether the TF binds to the probe, and then check the classification.
This suggested that although no ternary complex was visible, both proteins were bound to the probe simultaneously.
Being complementary, the gene product (messenger RNA) preferentially binds to the probe, cf. Figure 1.
Only the protein(s) bound to the probe is (are) radioactively labelled.
EMSA revealed that purified AtVOZ2 bound to the probe in a dose-dependent manner.
The recombinant PPARγ ligand binding domain protein bound tightly and specifically to this probe with Kd=61±14 nM as determined by FP measurements.
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