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The third EH domain displays a different specificity: FW containing motifs preferentially bind to this domain.
Since the C-terminal PDZ ligand domain is essential for inducing LTD, we considered molecules known to bind to this domain.
Interestingly, some nuclear import receptors also bind to this domain (Shah et al, 1998; Ben-Efraim et al, 2009) but relevance of these interactions in the context of Tpr's NPC attachment still remains unclear.
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The N-terminal domain has an ATP-binding site, and natural products such as geldanamycin and radicicol binds to this domain.
The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
FAD binds to this domain, near the N-terminal end of α1 and the carboxy ends of the strands β4 and β5.
An obvious candidate for the location of binding sites on VAMP7 for membrane traffic machinery is the longin domain, especially given the observation that AP3 binds to this domain (Martinez-Arca et al., 2003a, 2003b), as well as the demonstration that the regulatory Habc domain of the SNARE vti1b binds to the clathrin adaptor epsinR (Hirst et al., 2004; Miller et al., 2007).
In addition, studies by the Tedder laboratory have demonstrated that the CD22 ligand binding domain is required for in vivo B-cell survival [ 33], which could explain why anti-CD22 mAbs that bind to this ligand-binding domain have greater efficacy than anti-CD22 mAbs that bind to non-ligand-binding domains.
In this assay, isoforms with an unmasked TA domain bind to this external TID.
In addition to phospholipids, other ligands have been reported to bind this domain; however their binding properties remain uncharacterized.
In fact, this tyrosine motif is reported to bind to SH2 domain [ 45].
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