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Electrophoretic mobility shift assays (EMSA) and ChIP assays demonstrated that both Sp1 and Sp3 bind to these elements and the binding activity is enhanced in senescent cells.
A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter.
Hic-5 itself did not bind to these elements in a sequence specific manner, but p300 appeared to be involved in the induction of c-fos.
This chapter describes approaches and strategies for using minigenes to define the cis-acting elements that determine splice site usage and to identify and characterize the trans-acting factors that bind to these elements and regulate alternative splicing.
So far the identity of the transcription factors which bind to these elements is only known for the CHOP element (CHOP and C/EBPβ).
D'Erchia et al. recently reported that FASN harbors two p53 responsive elements (REs) in its promoter site, and that TAp73α, and ΔNp63α, but not p53, TAp73β or TAp63α directly bind to these elements and modulate expression of FASN at the transcriptional level [32].
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Transcription factors NFAT5 and Sp1, which are ubiquitously expressed in a variety of tissues, bound to these elements.
GT-4 formed a complex with P1 (GT-3 box), P2 (GT-3b box) and P7 (MRE4), and the intensity of the retarded bands were dramatically reduced when non-labeled competitors were included, indicating that GT-4 specifically binds to these elements.
The spacing between the cis elements determines the interactions between DBTFs that bind to those elements.
The AR can bind to these canonical elements, but promoter analysis of androgen-responsive genes revealed additional non-consensus response elements with apparent AR specificity.
β-catenin could bind to these response elements and suppress the expression of Inhbb in the fetal ovary.
More suggestions(15)
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com