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It has been estimated in a research study [ 24] that there might be 0 to 200 possible TGs for a TF in S. cerevisiae, though approximately only 3percentt of the TGs will have their binding sites bind to these TFs of S. cerevisiae.
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In such cases, co-regulators bind to the TFs prior to the DNA binding event.
Electrophoretic mobility shift assays (EMSAs) confirmed that three Drosophila mesodermal Fkh TFs bind to these sites (Fig. 1E-H).
Iron must be in its ferric form to bind to the Tf protein.
Thus, it is possible that the TFs that bind to these motif pairs interact with each other.
Identification of the TFs that bind to these motifs, and the characterization of identified, but unknown promoter elements will help elucidate the signaling pathways involved in the expression of these genes and potential involvement of metabolic regulation.
EMSA studies confirmed that a recombinant G-box specific basic leucine zipper (bZIP) TF can bind to these GREs [ 15, 19].
Cells take up Fe bound to Tf using Tf receptors (TfR); thus, the biological function of the specific receptors is to bind Tf on the cell surface and ingest it.
Iron-loaded Tf first binds to the Tf receptor (TfR) and enters the cell through clathrin-mediated endocytosis.
Binding to the TF-REs is independent of dimer phosphorylation.
The predicted TFBSs described previously are present in multiple copies within highly conserved regions across the Men locus and we suspected that TFs bound to these sites could regulate Men expression in cis, in addition to any role in trans-regulation.
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