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It is therefore most likely that Atf1 or Pcr1 can bind to their genomic targets without formation of heterodimer [22].
The ability of the IN-I-PpoI forms to bind to their genomic target sites was therefore next studied using chromatin ChIP-techniques.
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Transcription factors bound to their genomic target sequences may prevent DNA methylation at these sequences, whereas reduction or loss of transcription factor binding may result in de novo DNA methylation [ 18].
The Tet1 and Tet3 also contain a CXXC region, which can bind to the genomic CpG islands (Tan and Shi, 2012; Zhao and Chen, 2013).
However, CRISPR RNA is reported to bind to other genomic locations that differ from the intended target site by a few nucleotides, demonstrating significant off-target activity.
Subsequently, STAT proteins translocate to the nucleus and bind to conserved genomic regulatory sequences to rapidly activate gene transcription [42], [43].
GR can directly bind to the genomic GRE.
In many cases, TFs can bind to a genomic region at closely spaced adjacent sites.
We further demonstrated that Sp1 binds to MCRE, and that more Sp1 is localized in the nucleus at 32°C to bind to the genomic region containing MCRE.
We show that Tpk2 and to a lesser extent Tpk1 bind to specific genomic sequences within ORFs and promoters of target genes.
We identified numerous cases in which KLF4 tends to bind to those genomic regions containing a methylated CCCpGCC sequence, consistent with our protein microarray results.
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