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Antisense oligonucleotides bind to their complementary sequence on messenger RNAs and inhibit translation of the mRNA into the protein.
miRNAs are short, noncoding RNAs that usually bind to their complementary sequences in the 3'UTR of target mRNA, resulting in gene silencing or downregulation at the post-transcriptional level [28].
SNP assays rely on the biochemical principle that nucleotide bases bind to their complementary bases (adenine binds to thymine and cytosine binds to guanine) [ 7].
PNAs recognize and bind to their complementary nucleic acid sequences with higher thermal stability and specificity than the corresponding oligodeoxyribonucleotides; this was exploited to develop Light-up probes for microchip applications and detection of PCR products [ 17].
When ASOs are introduced into cells, they bind to their complementary target mRNA and cause their degradation through the activity of RNase H or they inhibit the mRNA utilization and translation into proteins.
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The 3DNA capture reagents bound to their complementary cDNA capture sequences on the oligo d(T) primers.
MicroRNAs (miRNAs) are endogenous 20- to 25-nucleotide-long non-coding RNAs that bind to their targets through partial sequence complementary within the 3′-untranslated region (UTR) or open reading frame (ORF) of coding mRNAs.
MicroRNAs (miRNAs) are small noncoding RNAs that bind to complementary sequences in their target mRNAs and either block their translation or promote their turnover (Bartel, 2004, 2009).
As miRNAs bind to partially complementary sequences in their target mRNAs, computational analysis can be used to predict targets [ 33, 34].
They bind to partially complementary sites in the messenger RNAs (mRNAs) of their target genes, thereby inducing the post-transcriptional mechanisms of gene silencing [ 1].
These fragments can bind to complementary transcripts and downregulate their expression via an RNA interference-like mechanism, as explained above.
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