As mentioned above, water-insoluble lipophilic compounds are always transported bound to their binding proteins and the technique described here profits of the very strong binding of such lipophilic ligands to ghosts filled with their binding proteins.
We herein also adopted a similar approach to test whether and how CupB2/CupB4 bind to their putative binding partners.
Transcriptional regulation, a primary mechanism for controlling the development of multicellular organisms, is carried out by transcription factors (TFs) that recognize and bind to their cognate binding sites.
A general question is how motors search for sites in the cell where both motor ends can bind to their respective binding partners.
To investigate the effect of binding of the ions and the substrate on protein dynamics, one would ideally design and perform equilibrium MD simulations of the protein in the presence of these species initially placed in the bulk and monitor their movement over time as they diffuse, find, and bind to their respective binding sites.
These transcription factors bind to their respective putative binding sites and initiate the transcription of type I IFN, which activates auto- or paracrine pathways to initiate the antiviral responses.
Intracellular protein protein interactions (PPIs) are challenging targets for conventional drug modalities, because small molecules generally do not bind to their large, flat binding sites with high affinity, whereas monoclonal antibodies cannot cross the cell membrane to reach the targets.
Furthermore, TRAF3 and TRAF6 independently bind to their own specific binding sites in IPS-1 [25], [25].
With what affinity do growth factors bind to their receptors or binding proteins that enhance or attenuate their function?
After degradation of its cytoplasmic inhibitor IκB, the NF-κB proteins translocate into the nucleus and bind to their cognate DNA binding sites to regulate transcription of a large number of genes.
