Sentence examples for bind to the template from inspiring English sources

Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is.

In case of mutation(s), PCR efficiency will be extremely reduced due to the presence of additional mismatch es) at the 3′ end of the MAMA primer which will not bind to the template; thus, amplification of the target gene is failed [ 27].

Primers were allowed to bind to the template RNA at 70° for 5 min and the reaction proceeded at 25° for 10 min, 37° for 60 min, and 85° for 10 min. RT-qPCR reactions were carried out in a CFX384 Real-Time PCR Detection System (Bio-Rad).

For all cycles, annealing was performed for 1 min, denaturation at 95°C for 20 sec and extension at 72°C for 1 min, followed by a final extension at 72°C for 5 min. To facilitate direct sequence analysis of PCR products from bisulfite-treated templates, GC-tagged primers were used containing additional nucleotides at the 5' end (indicated by bold letters) that do not bind to the template.

These interactions may also serve as a wedge to displace a complementary oligonucleotide fragment bound to the template in front of the growing DNA chain that may impede chain elongation.

The primer annealing temperature determines that the primer completely binds to the template or partially mismatches the template in one or several bases.

This short inserted sequence in general is not expected to base pair to the template strand, but rather loops out when the primer is bound to the template.

Similarly, as a control reaction, the single-stranded 120 bp DNA was amplified by conventional PCR in which the first primer (PCR-1) bound to the template DNA in an antiparallel orientation and the second primer (PCR-2) annealed to the newly synthesized DNA in an antiparallel orientation.

Even if it turns out that any polymerase ribozyme has to be very long, the preliminary replicase ribozyme may have only been a ligase – short enough and loosely bound to the template-substrate complex – as demonstrated in our computer simulation work concerning the replicase [ 18] (actually just being the Rep in the current model).

During the initial five to eight cycles of amplification, the defined and variable nucleotides at the 3′-end of the primers bind to the DNA template at many sites throughout the genome, followed by strand extension.

However, as shown in Table 1, the net inhibition of all polymerases when the drugs were added after the RNA polymerase was allowed to bind to the primer template was very limited because the inhibition plateaued at 43 73% even at high drug concentrations.