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Overall, this work illustrates how virtual screening can identify a diverse set of ligands which bind to the targeted site.
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However, for detection in cells, the complementary oligonucleotide probes are expected to bind to the targeting mRNA and the nonspecific BTO monomers are washed away.
The conclusion was that MYC actually requires certain modifications to bind to the target E box and that binding is a signal for further modifications.
Next, GSH-bound PrfA will bind to the target DNA to regulate gene transcription.
These results indicate that antibody-PFC/QD nanoemulsions selectively bind to the target-protein.
Finally, PPI targets usually lack natural molecules that bind to the target PPI interface.
Due to the high sensitivity of PET, such radiotracers must bind to the target protein with high selectivity.
DNA-based recognition elements, aptamers, whose function is analogous to antibodies, bind to the target and generate signal of recognition.
Aptamers are kinds of synthetic peptide or oligonucleotide molecules which can bind to the target molecule firmly.
The antibodies attached on the surface of the nanoparticles selectively bind to the target molecule with high affinity.
The principal disadvantage of a ligand-based approach is the need for a priori knowledge of a set of ligands known to bind to the target [7].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com