Sentence examples for bind to the primary from inspiring English sources

Briefly, depletion of CD4-negative T cells was achieved by incubation of peripheral blood mononuclear cells with a cocktail of monoclonal antibodies directed against non-T cells and against CD4-negative T cells, followed by binding of a secondary microbead-coupled antibody to bind to the primary antibodies.

Furthermore, we demonstrate that HIV and SIV Nef bind to the primary viral receptor CD4 with comparable efficiency.

The embryos were also incubated with commercially available secondary antibodies that bind to the primary antibodies, and that are cross-linked to fluorescent dyes (Alexa488, Alexa555 and Alexa647).

Species-specific secondary antibodies, called PLA probes, each with a unique short DNA strand attached to it, bind to the primary antibodies.

Thus, a collagen-like peptide with only two GVMGFO motifs may be unable to activate DDR on cells, even though it would still bind to the primary site at the top of the DS domain.

The membranes were then further incubated with infrared- labeled secondary antibodies (donkey anti-rabbit IRDye 800, Li-Cor Biosciences, Lincoln, NE) added to bind to the primary antibody at room temperature for 1 hr.

Simberg and co-workers introduced a model for the protein corona which includes "primary binders" that interact with the NPs surface at first followed by "secondary binders" that binds to the primary binders by way of protein protein interactions [39].

DNase I footprinting analysis of DevRC with the tgs1-Rv3131 interegion revealedevealed that it binds to the primary site, but fails to cooperatively bind to the adjacent site, unlike full-length DevR which protected both the sites (Fig. 2).

The secondary antibody Goat Anti-Mouse IgG AP conjugate bound to the primary antibody (IgG) and the signal was enhanced.

After incubation and washing, strong magnets were used to remove all beads that bound to the primary or secondary antibody.

The protein bands specifically bound to the primary antibodies were detected using an anti-rabbit IgG alkaline phosphatase-linked secondary antibody and an ECF (enhanced chemifluorescence) Western blot analysis system (Amersham Pharmacia Biotech, Piscataway, NJ, USA) (Ouyang et al. 2006).